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hpv 18  (ATCC)


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    Structured Review

    ATCC hpv 18
    Hpv 18, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hpv+18+plasmid/HPV+type+18%3B+purified+plasmid+DNA/pmc12377189-44-4-6
    Average 93 stars, based on 2 article reviews
    hpv 18 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    other:

    Article Title: Self-Digitization Chip for Quantitative Detection of Human Papillomavirus Gene Using Digital LAMP
    Article Snippet: Chemicals and reagents: Bst 2.0 WarmStart DNA Polymerase and Isothermal Amplification Buffer were purchased from New England BioLabs (Ipswich, MA).

    Sequencing:

    Article Title: Identification of a novel promoter in the E2 open reading frame of the human papillomavirus type 18 genome.
    Article Snippet: Northern blot and RT-PCR analyses indicated that the human papillomavirus E4 open reading frame is expressed in HeLa cells.. Because integration at the E1 or E2 open reading framewould place the viral upstream regulatory region downstreamof theviral lategenes, theexpressionofE4 in HeLa cells is most likely regulated by host cellular promoter(s) or unidentified viral promoter(s) in the E2 region.. Primer extension analysis and transient transfection experiments with luciferase reporter constructs under the transcriptional control of various subgenomic fragments of HPV-18 were carried out to identify and characterize functional promoters within the E2 region.

    Plasmid Preparation:

    Article Title: Identification of a novel promoter in the E2 open reading frame of the human papillomavirus type 18 genome.
    Article Snippet: Northern blot and RT-PCR analyses indicated that the human papillomavirus E4 open reading frame is expressed in HeLa cells.. Because integration at the E1 or E2 open reading framewould place the viral upstream regulatory region downstreamof theviral lategenes, theexpressionofE4 in HeLa cells is most likely regulated by host cellular promoter(s) or unidentified viral promoter(s) in the E2 region.. Primer extension analysis and transient transfection experiments with luciferase reporter constructs under the transcriptional control of various subgenomic fragments of HPV-18 were carried out to identify and characterize functional promoters within the E2 region.

    Article Title: Self-Digitization Chip for Quantitative Detection of Human Papillomavirus Gene Using Digital LAMP
    Article Snippet: Light mineral oil, betaine, bovine serum albumin (BSA), and MnCl 2 were purchased from Sigma-Aldrich (St. Louis, MO). .. HPV-18 plasmid (full-genome length, 45152D) was purchased from American Type Culture Collection (Manassas, VA). ..

    Derivative Assay:

    Article Title: Identification of a novel promoter in the E2 open reading frame of the human papillomavirus type 18 genome.
    Article Snippet: Northern blot and RT-PCR analyses indicated that the human papillomavirus E4 open reading frame is expressed in HeLa cells.. Because integration at the E1 or E2 open reading framewould place the viral upstream regulatory region downstreamof theviral lategenes, theexpressionofE4 in HeLa cells is most likely regulated by host cellular promoter(s) or unidentified viral promoter(s) in the E2 region.. Primer extension analysis and transient transfection experiments with luciferase reporter constructs under the transcriptional control of various subgenomic fragments of HPV-18 were carried out to identify and characterize functional promoters within the E2 region.

    Clone Assay:

    Article Title: Identification of a novel promoter in the E2 open reading frame of the human papillomavirus type 18 genome.
    Article Snippet: Northern blot and RT-PCR analyses indicated that the human papillomavirus E4 open reading frame is expressed in HeLa cells.. Because integration at the E1 or E2 open reading framewould place the viral upstream regulatory region downstreamof theviral lategenes, theexpressionofE4 in HeLa cells is most likely regulated by host cellular promoter(s) or unidentified viral promoter(s) in the E2 region.. Primer extension analysis and transient transfection experiments with luciferase reporter constructs under the transcriptional control of various subgenomic fragments of HPV-18 were carried out to identify and characterize functional promoters within the E2 region.



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    Strains used in this study.

    Journal: Journal of Microbiology and Biotechnology

    Article Title: Development of a Novel Multiple Cross-Linking Spiral Amplification for Rapid and Sensitive Detection of HPV16 DNA

    doi: 10.4014/jmb.2012.12047

    Figure Lengend Snippet: Strains used in this study.

    Article Snippet: pHPV-18 , ATCC 45152D , - , - , -.

    Techniques: Synthesized